Laboratory # 1 Examination
What is living in your mouth?
In this laboratory test you will examine your mouth microflora by Gram staining.
During Lab
Prepare a teeth/gum sample slide smear by following instruction.
Perform Gram stain on your teeth/gum sample by following instruction.
Examine your slide under the microscope at 100X and pick an area with diverse bacteria.
Take a picture or draw a sketch of the bacteria morphology and arrangement you found in your slide. (This must be included in your report next week)
Label your slide with your sample name (teeth/gum) and initials. Save your slide in the slide box.
Laboratory Report
Introduction
Background on importance of understanding our mouth microbiota.
How did you examine your mouth microflora in this laboratory exercise?
Why was Gram staining used in this exercise and not simple staining?
Results
Include a picture of your slide or a picture of your sketch with labels (Include figure legend)
Create a table to indicate morphology, arrangement, and potential presumptive IDs of your bacteria (see example below)
Bacteria observed under 100X
Gram-stain result
Morphology
Arrangements
Presumptive IDs (Genus Species)
Purple cells
(Gram-positive)
Spherical (cocci)
Single
Diplococcus
Streptococcus
Streptococcus mutans
Streptococcus mitis Streptococcus gordonii
Discussion
Based on the presumptive ID’s, what type of microflora do you think you have (ie healthy or diseases causing)? Explain your answer.
Based on the presumptive ID’s which organisms cause periodontal disease and which if any are considered protective for health? (Give at least 3 examples)
Do periodontal disease bacteria cause other diseases? (Give 2 disease examples)
What did this exercise teach you about your oral health? Are you considering any changes in your oral care routine? Why or why not?
Report format
Your report should be at least 3-4 pages not including citations.
Font: Times New Roman
Size: 12
1.5 spaced and 1” margins.
APA citation format
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